CHARACTERIZATION AND EVALUATION OF SECONDARY METABOLITES FROM IPOMOEA BATATAS VAR. CILEMBU BARK: EXTRACTION, ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL SCREENING

Authors

  • Fathatul Akrami Universitas Negeri Padang
  • Ronal Deniro Universitas Negeri Padang
  • Syifa Syakirah Universitas Negeri Padang
  • Fitri Amelia Universitas Negeri Padang
  • Hesti Parbuntari Universitas Negeri Padang
  • Fazila Ameliya Universitas Negeri Padang
  • Latifa Permata Universitas Negeri Padang
  • Welni Rahmadani Universitas Negeri Padang

DOI:

https://doi.org/10.20414/spin.v8i1.15155

Keywords:

Phytochemistry, Antioxidants, Extraction, Evaluation, Characterization

Abstract

This study aimed to evaluate the antioxidant potential and secondary metabolite content of Cilembu sweet potato (Ipomoea batatas var. cilembu) peel using maceration and reflux extraction methods. The extraction process used 70% ethanol as a solvent, followed by phytochemical analysis, antioxidant activity using the DPPH method, and characterization using TLC and FTIR. The results showed that the maceration and reflux extracts contained flavonoids, phenolics, terpenoids, and saponins. Antioxidant activity was expressed as an IC50 value, where the reflux extract had an IC50 of 244,5 ppm, lower than the maceration extract with an IC50 of 86,776  ppm, indicating stronger antioxidant activity. FTIR analysis identified functional groups consistent with the main bioactive compounds. This study confirmed the potential of Cilembu sweet potato peel as an effective source of natural antioxidants with higher efficiency through the reflux method compared to maceration.

 

ABSTRAK

Penelitian ini bertujuan untuk mengevaluasi potensi antioksidan dan kandungan metabolit sekunder dari kulit ubi Cilembu (Ipomoea batatas var. cilembu) menggunakan metode ekstraksi maserasi dan refluks. Proses ekstraksi menggunakan etanol 70% sebagai pelarut, diikuti dengan analisis fitokimia, aktivitas antioksidan dengan metode DPPH, serta karakterisasi menggunakan KLT dan FTIR. Hasil penelitian menunjukkan bahwa ekstrak maserasi dan refluks mengandung flavonoid, fenolik, terpenoid, dan saponin. Aktivitas antioksidan dinyatakan dalam nilai IC50, di mana ekstrak refluks memiliki IC50 sebesar 244,5 ppm, lebih rendah dibandingkan dengan maserasi dengan IC50 86,776  ppm, menunjukkan aktivitas antioksidan yang lebih kuat pada ekstrak maserasi. Analisis FTIR mengidentifikasi gugus fungsi yang konsisten pada senyawa bioaktif utama. Penelitian ini mengkonfirmasi potensi kulit ubi Cilembu sebagai sumber antioksidan alami yang efektif dengan efisiensi yang lebih tinggi melalui metode refluks dibandingkan maserasi

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Published

2026-07-07

Issue

Vol. 8 No. 1 (2026): January - June 2026

Section

Articles

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